Guo and Lee use computational and synthetic biology approaches to develop a new method called SunRISER for visualizing single mRNA molecules in living cells. By observing cells over timescales of a full day, they show that environmental stresses lead to heterogeneity between genetically identical sister cells through asymmetric mitotic mRNA inheritance. They argue that asymmetric inheritance can rapidly diversify individuals among cancer cell populations leading to robustness against harsh and nutrient-limited environments.
Guo Y, Lee REC (2022) Long-term imaging of individual mRNA molecules in living cells. Cell Reports Methods. 100226